Projects
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Allan Brown, a Ph.D.candidate in
the Department of Natural
Resources and Environmental Sciences under Dr. Jack Juvik, was the
first
user of the Center. His research involved the molecular mapping of
glucosinolate production in broccoli using SSR markers. |
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Dr. Randall Nelson from the USDA is
directing a project to
investigate protein concentration in soybeans. He says: "We are mapping
seed protein concentration in three F3 BC2
populations. Each population has Williams 82 as the recurrent
parent and
three different sources of high protein as the donor parents. The
original
sources of high protein are PI 82.278, Sioux, and HHP. HHP was
developed
by Henry Hadley in Crop Sciences from an unknown G. soja line.
The BC1
parental lines had protein concentrations that ranged from 48 to 51%.
We
will be identifying loci associated with high protein concentration and
determining if there are different loci in different sources." |
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The Brian Diers lab performs
molecular marker screens each
year on potential parents to be used in crosses, and lines segregating
for SCN resistance. The parental screening project has been done with a
battery of SSR markers on selected germplasm. This screening process
helps to identify those lines with the greatest dissimilarity in their
marker phenotypes and helps to determine the appropriate crosses to be
made in the field. This screening is done in an attempt to help our
crosses generate greater amounts of genetic diversity in breeding
material and is of value due to the notoriously narrow genetic base of
commercial soybeans. |
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The Jon Beever lab is using the
Center to conduct a genome
wide scan for meat quality quantitative trait
loci (QTL) in swine. This project is the largest one of it's kind being
done in swine. Once identified, these markers will be used in marker
assisted selection in breeding programs
in an effort to improve meat quality and the consistency of meat
quality in swine lines. |

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"Cooperative
breeding in
bluegill sunfish, Lepomis macrochirus: A survey of genetic
relatedness." Bluegill parental males display cooperative
breeding during spawning in large nest colonies at different
locations. I am interested in whether these parental males are
related to one another, thereby conveying a genetic benefit by doing
so. Using 6 different polymorphic loci to get a "genetic
fingerprint" from 450 bluegill, I am determining if these fish are in
fact related. Natasha Silich, Graduate Research Assistant, and
John Epifanio, INHS.
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“Microsatellite
DNA markers are
used
to generate multi-locus genotypes for three life stages of largemouth
bass; larval, juvenile, and adult. These DNA ‘fingerprints’ are
used to determine parentage and nest origin of juvenile fish. The
goal is to investigate the degree and sources of variance in
nest-specific contribution to recruitment.”
-Joe Parkos, PhD candidate Department
of Animal Biology
David Phillip and David Whal, INHS (CAE), Animal
Biology
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